Journal: eLife
Article Title: Transcription factor clusters regulate genes in eukaryotic cells
doi: 10.7554/eLife.27451
Figure Lengend Snippet: ( A ) Zoom-in on pairwise difference distribution for stoichiometry of GFP-labeled Mig1 foci detected during FRAP, ~8 mer intervals (dashed lines) and power spectrum (inset) shown, mean and Gaussian sigma error (arrow). ( B ) GFP-labeled Mig1 cluster size as a function of stoichiometry with power law fit indicated. ( C ) Immuno-gold transmission electron microscopy for negatively stained 90 nm cryosections of (upper panel) two different Mig1-GFP cells and (lower panel) two different Msn2-GFP cells, with zoom in (inset).
Article Snippet: Stoichiometry distributions were rendered as objective kernel density estimations ( ) using a Gaussian kernel with bandwidth optimized for normally distributed data using standard MATLAB routines.
Techniques: Labeling, Transmission Assay, Electron Microscopy, Staining